Activity-dependent genetic dissection of LS circuits for reward and punishment

The LS is potently stimulated and modified in a long-lasting manner by exposure to stressful stimuli, drugs of abuse, and drug withdrawal. However, the relationships between the LS neuronal subpopulations that control behavioral responses to rewarding or aversive stimuli are largely undefined. We have developed and are employing a novel system for activity-dependent genetic marking to specifically target reward- and punishment-responsive neurons for functional analysis via in vivo imaging, electrophysiological recordings, and optogenetic manipulations.

Viral-mediated Tetracycline-regulated Activity-dependent Circuit Marking (TRACM) in the LS